mix gas6 (MedChemExpress)

MedChemExpress mix gas6

(A) Binding of different antibodies to AXL. Binding of different antibodies to AXL and their interaction with <t>Gas6.</t> (B) List of full length and different truncations of AXL. (C) αAXL-9# and αAXL-13# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations. (D) αAXL-4# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations with or without Gas6-his.

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(A) Binding of different antibodies to AXL. Binding of different antibodies to AXL and their interaction with Gas6. (B) List of full length and different truncations of AXL. (C) αAXL-9# and αAXL-13# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations. (D) αAXL-4# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations with or without Gas6-his.

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: (A) Binding of different antibodies to AXL. Binding of different antibodies to AXL and their interaction with Gas6. (B) List of full length and different truncations of AXL. (C) αAXL-9# and αAXL-13# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations. (D) αAXL-4# were analyzed via flow cytometry for reactivity towards hAXL or hAXL truncations with or without Gas6-his.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Binding Assay, Flow Cytometry

The binding characteristic of AXL-4#. (A) Representation of interplay of AXL antibody and Gas6. (B) 293T-hAXL cells were incubated with isotype control (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (C) 293T-memGas6 cells were incubated with anti-Gas6l (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (D,E) The structure of αAXL-4# recognizing Gas6/AXL complex predicted by AlphaFold.

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: The binding characteristic of AXL-4#. (A) Representation of interplay of AXL antibody and Gas6. (B) 293T-hAXL cells were incubated with isotype control (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (C) 293T-memGas6 cells were incubated with anti-Gas6l (left), AXL-4# (middle) or AXL4# with Gas6 (right), then stained with PE-goat anti mice IgG2a. (D,E) The structure of αAXL-4# recognizing Gas6/AXL complex predicted by AlphaFold.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Binding Assay, Incubation, Control, Staining

Application of αAXL-4# in flow cytometry. (A,B) The binding of αAXL-4# to Gas6/AXL complex of different Species. (C,D) asDCs and other cell lines were incubated with αAXL-9#, αAXL-13 and αAXL-4#.

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: Application of αAXL-4# in flow cytometry. (A,B) The binding of αAXL-4# to Gas6/AXL complex of different Species. (C,D) asDCs and other cell lines were incubated with αAXL-9#, αAXL-13 and αAXL-4#.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Flow Cytometry, Binding Assay, Incubation

Application of αAXL-4# in ELISA. (A) Different concentrations of AXL (0-1 μg/mL) with a single dose of Gas6 (0.25 μg/mL, red column) or without Gas6 (empty column) were added subsequently. (B) Different concentrations of Gas6 (0–1 μg/mL) with a single dose of AXL (1 μg/mL, red column) or without AXL (empty column) were added subsequently.

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: Application of αAXL-4# in ELISA. (A) Different concentrations of AXL (0-1 μg/mL) with a single dose of Gas6 (0.25 μg/mL, red column) or without Gas6 (empty column) were added subsequently. (B) Different concentrations of Gas6 (0–1 μg/mL) with a single dose of AXL (1 μg/mL, red column) or without AXL (empty column) were added subsequently.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Enzyme-linked Immunosorbent Assay

Different levels of AXL-Gas6, AXL and Gas6 in human plasma. (A–C) Plates were coated by AXL-4# (A) , commercial AXL antibody (B) or commercial Gas6 antibody (C) respectively. The levels of AXL-Gas6, AXL, or Gas6 in human plasma were detected by ELISA.

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: Different levels of AXL-Gas6, AXL and Gas6 in human plasma. (A–C) Plates were coated by AXL-4# (A) , commercial AXL antibody (B) or commercial Gas6 antibody (C) respectively. The levels of AXL-Gas6, AXL, or Gas6 in human plasma were detected by ELISA.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay

ROC curve and association with AXL/AXL-Gas6 with clinical data. (A) ROC curve; (B) AXL was associated with Cr in STEMI; (C–G) Gas6/AXL complex was associated with PLT, WBC, HDL, CK-MB and SO-FMC as indicated.

Journal: Frontiers in Medicine

Article Title: Detection of Gas6/AXL complex and its expression changes in patients with ST-segment elevation myocardial infarction

doi: 10.3389/fmed.2025.1653708

Figure Lengend Snippet: ROC curve and association with AXL/AXL-Gas6 with clinical data. (A) ROC curve; (B) AXL was associated with Cr in STEMI; (C–G) Gas6/AXL complex was associated with PLT, WBC, HDL, CK-MB and SO-FMC as indicated.

Article Snippet: As previously reported , mix Gas6 (250 ng/mL, HY- P70470 , MCE) with AXL (30 ng/mL, DY154, R&D Systems) in a total volume at 20 μL for 1 h to obtain the AXL-Gas6 complex.

Techniques:

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